KELLY R. ZAMUDIO: RESEARCH PROGRAM

My research interests lie in the fields of population biology, systematics and character evolution. I am particularly interested in the links between patterns of genetic differentiation and attributes of the ecology and life history of organisms such as mating systems, dispersal and demography. In my research I combine field and laboratory (molecular) approaches to answer questions about organisms in an integrated framework.

     
   

My research can be divided into three broad areas of investigation: 1) studies of mating system and sexual selection, 2) phylogenetic systematics of populations or species of reptiles and amphibians, and 3) application of my basic research to conservation. The conceptual thread that ties these together is my interest in disentangling the relative roles of organismal attributes and microevolution in shaping phylogenetic relationships among populations or species. Thus, in my studies of mating systems and sexual selection, I have focused on characterizing the determinants of reproductive success, with the goal of understanding the evolutionary consequences of large disparities in fitness within breeding groups. For instance, high variance in reproductive success among individuals and fluctuations in population size over time are two factors that can greatly influence the genetic effective population size and hence genetic drift. Population parameters such as these can be tied closely to organismal traits, so ecology, behavior, and demography at the individual level can have important effects on overall patterns of population structure at larger spatial scales. I relate my findings in the study of mating systems to patterns of population differentiation at multiple geographic scales ranging from local genetic variation among populations, to rangewide genetic variation and phylogenetic relationships among groups of species. Finally, I take every opportunity to apply my findings to the conservation genetics and management of amphibians and reptiles.

Genetic characterization of mating systems and sexual selection
The diverse array of mating systems exhibited by reptiles and amphibians presents an ideal opportunity to examine the selective costs and benefits of mating strategies and choices. Despite the diversity of mating systems and the behavioral intricacies of ectotherm reproductive behavior, sexual selection in ecothermic lineages has not received the same degree of attention as in endotherms. Certain life history traits such as large clutch sizes, inaccessibility of nests or breeding groups, and primarily nocturnal behaviors have historically limited studies of mating systems and sexual selection in many ectothermic lineages. This “clade bias” is changing rapidly. Over the last decade we have seen an increased emphasis on the study of behavior, reproductive success, and kinship in all animal lineages. The absence of extended parental care in most ectotherms makes it easier to estimate the cost-benefit ratio of mate choice. Likewise, it is now feasible to establish reproductive success (via paternity typing) of large numbers of offspring, allowing us to accurately quantify the outcome of male-male competition and female choice (intra- and inter-sexual selection).


My research has focused particularly on the following questions, with the overall goal of understanding the diversity and evolution of mating systems in these lineages:

  1. What are the ecological and evolutionary contexts for the evolution of alternative mating strategies?
  2. What are the determinants of reproductive fitness, especially in systems with high variance in reproductive success?
  3. What are the population-level consequences (genetic) of high variance in reproductive success?

My goal is to design experiments in the lab and in the field to test hypotheses about mate choice, reproductive success, and fitness. I use field experiments and behavioral observations coupled with laboratory studies for molecular assessment of paternity and maternity rates in natural populations. As is often the case in science, I have been surprised by my results on a number of occasions, underscoring the importance of exploring a variety of mating systems before drawing general conclusions about their evolution.


Phylogenetic systematics: population differentiation, phylogeography, and species relationships
I extend my studies of kinship, relatedness and family genealogies with phylogenetic studies of diversification among populations, lineages, and species. I focus on microevolutionary processes that contribute to differentiation among populations, with particular attention on how species-specific traits (such as demography, dispersal capacity, reproductive skew) influence the rate or direction of differentiation. In systematic studies at larger geographic scales I focus on the patterns and inferred processes of historical diversification among populations and species.

  1. How do limits to dispersal and changes in habitat availability (due to past climatic changes) interact to determine the pattern of evolutionary diversification of population lineages?
  2. Do co-occuring species exhibit similar patterns of population-level diversification (i.e. similar phylogeographic patterns)?
  3. How do species- specific characteristics (such as life-history, behavior, mating system) influence phylogeographic patterns.
    As lineages diversify, what mechanisms play a role in reproductive isolation and eventually speciation?
  4. Once speciation has occurred, resulting in reciprocally monophyletic lineages, can we reconstruct from phylogenies the modes and patterns of differentiation by examination of neontological data?

Conservation genetics of reptiles and amphibians
It is probably impossible for an organismal biologist to conduct research without consideration of the threats to habitats and the flora and fauna that occupy them. I have found myself repeatedly intrigued by the implications of my research for the management of endangered or threatened species. Thus far, I have focused primarily on conservation genetics of individual threatened taxa and on how science is used in policies and recovery actions for these taxa. In the near future I hope to extend my use of population genetic methods to inform management and conservation decisions.


In my conservation efforts I focus on the following questions:

  1. How is genetic variability partitioned among populations of endangered or threatened species that inhabit patchy or fragmented landscapes?
  2. Does fragmentation impede historical levels of population connectivity through limits on gene flow, and if so, at what scale?
  3. Do mating systems (especially those resulting in high variance in reproductive success) exacerbate problems associated with inbreeding and reduced genetic variability in small isolated populations of threatened taxa?
     
   

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